Diagnostic Immunohistochemistry E-Book , livre ebook

Instructions for online access
Thank you for your purchase. Please note that your purchase of this Elsevier eBook also includes access to an online version. Please click here (or go to http://ebooks.elsevier.com ) to request an activation code and registration instructions in order to gain access to the web version.

Diagnostic Immunohistochemistry
Theranostic and Genomic Applications
Sixth Edition

David J. Dabbs, MD
Professor of Pathology (Emeritus), University of Pittsburgh Medical School, Pittsburgh, Pennsylvania
Chief of Pathology, PreludeDx, Laguna Hills, California
Table of Contents
Instructions for online access
Cover image
Title page
Copyright
Dedication
Contributors
Preface
How to Use This Book
1. Techniques of Immunohistochemistry: Principles, Pitfalls, and Standardization
Overview
Basic Principles of Immunohistochemistry
Antibodies as Specific Reagents for Detection of Proteomic Targets
Blocking Nonspecific Background
Detection Systems
Quality Control and Standardization
Tissue Fixation, Processing, and Antigen Retrieval Techniques
Techniques, Protocols, and Troubleshooting
Future Directions: Companion Diagnostics Present New Challenges for Immunohistochemistry
Conclusion
2. Quality Assurance in Immunohistochemistry in the Age of Precision Medicine
Introduction
Critical Definitions
What Is Quality in Immunohistochemistry?
Immunohistochemistry as a Qualitative Methodology in Precision Medicine
The Importance of Purpose in Immunohistochemistry Biomarker Assays
Validation of Immunohistochemistry Assays in Precision Medicine
The Role of External Quality Assurance Proficiency Testing
3. Immunohistology of Infectious Disease
Introduction
Viral Infections
Bacterial Infections
Fungal Infections
Protozoal Infections
Emerging Infectious Diseases
Pathologists, Immunohistochemistry, and Bioterrorism
Beyond Immunohistology: Molecular Diagnostic Applications
4. Immunohistology of Neoplasms of Soft Tissue and Bone
Overview
Biology of Antigens and Antibodies
Specific Soft Tissue and Bone Tumors
Summary
5. Immunohistology of Bone Marrow, Spleen, and Histiocytic Disorders
Overview
Bone Marrow Antigens
Normal Bone Marrow: Immunohistochemical Identification of Normal Components
Myeloid Diseases
Mastocytosis
Spleen
Histiocytic and Dendritic Neoplasms
Summary
6. Immunohistology of Lymph Node and Lymph Node Neoplasms
Antigens for Evaluation of Hematologic Disorders
Establishing the Diagnosis of Lymphoma: Differential Diagnostic Considerations
Immunohistochemical Evaluation of Small B-Cell Lymphomas
Extranodal Marginal Zone Lymphoma
Large B-Cell Lymphomas and Other Aggressive B-Cell Lymphomas
Large B-Cell Lymphoma Subtypes
T-Cell Lymphomas
Hodgkin Lymphoma
Pitfalls in the Diagnosis of Lymphoma: Mimicry
Summary
7. Immunohistology of Melanocytic Neoplasms
Overview
Biology of Antigens and Antibodies
Neuroendocrine Markers in Melanocytic Lesions
Sentinel Lymph Node Biopsies for Metastatic Melanoma
Application of Immunohistochemistry to Selected Differential Diagnoses
Prognostic Markers and Targeted Therapy for Melanoma
Summary
8. Immunohistology of Carcinoma of Unknown Primary Site
Cancer of Unknown Primary Site: Clinical Aspects and Economic Considerations
Diagnostic Approach to the Study of Cancer of Unknown Primary Site: Specimen Preparation
Determining Site of Origin: Stepwise Approach
Combined Antibody (Panel) Approach to Solving Diagnostic Problems
Special Clinical Presentations
Molecular Approach for Determining Site of Origin
Summary
9. Immunohistology of Head and Neck Lesions
Introduction
Squamoproliferative Lesions
Nasal Cavity and Paranasal Sinuses
Nasopharynx
Oral Cavity
Larynx/Hypopharynx
Salivary Glands
Ear and Temporal Bone
Metastatic Tumors
Summary
10. Immunohistology of Endocrine and Neuroendocrine Neoplasms
Overview
Biology of Antigens and Antibodies
Tumors of Specific Sites
Neuroendocrine Tumors in Other Sites
Summary
11. Immunohistology of the Mediastinum
Introduction
The Normal Thymus
Thymic Tumors
Thymic Epithelial Tumors
Thymic Carcinoma
Germ Cell Tumors
Mediastinal Sarcomas
Neurogenic and Neuroectodermal Neoplasms
Lymphoproliferative Tumors
Summary
12. Immunohistology of Pulmonary and Pleural Neoplasms
Malignant Epithelial Neoplasms of the Lung
Biphasic Neoplasms of the Lung
Neuroendocrine Tumors of the Lung
Mesenchymal Tumors of the Lung
Primary Vascular Neoplasms of the Lung
SMARC—Deficient Neoplasms
Unusual Primary Tumors of the Lung
Lymphoproliferative Tumors of the Lung
Tumors of the Pleura
13. Immunohistology of Skin Tumors
Overview
Epithelial Tumors of the Skin
Cutaneous Lymphohematopoietic Disorders
Mesenchymal Tumors of The Skin
Special Topics in Cutaneous Immunohistochemistry
14. Immunohistology of the Gastrointestinal Tract
Biology of Antigens and Antibodies: General and Tissue Specific
Diagnostic Immunohistochemistry: Epithelial Lesions of the Gastrointestinal Tract
Diagnostic Immunohistochemistry: Neuroendocrine Neoplasms of the Gastrointestinal Tract
Diagnostic Immunohistochemistry: Mesenchymal Lesions of the Gastrointestinal Tract
Beyond Immunohistochemistry: Theranostic and Genomic Applications
Summary
15. Immunohistology of Pancreas and Hepatobiliary Tract
Pancreas
Extrahepatic Biliary Tract (Extrahepatic Bile Ducts and Gallbladder)
Ampulla
Liver
Summary
16. Immunohistology of the Prostate
Overview
Biology of Antigens and Antibodies
Diagnostic Immunohistochemistry of Specific Prostate Lesions
Beyond Immunohistochemistry: Theranostic and Genomic Applications
Summary
17. Immunohistology of the Bladder, Kidney, and Testis
Overview
Immunohistology of the Urinary Bladder
Immunohistology of Renal Neoplasms
Immunohistology of Testicular Tumors
Summary
18. Immunohistology of the Female Genital Tract
Biology of Antigens and Antibodies
Lower Female Genital Tract and Uterus
Upper Female Genital Tract
Summary
19. Immunohistology of the Breast
Introduction
Myoepithelial Cells and Assessment of Stromal Invasion
Immunohistochemistry of Papillary Lesions
Proliferative Ductal Epithelial Lesions and In Situ Carcinoma
Tumor Type Identification by Immunohistochemistry
Immunohistochemistry for Identifying Special Types of Breast Carcinomas
Paget Disease of the Breast
Detection of Lymphatic Space Invasion
Sentinel Lymph Node Examination
Systemic Metastasis of Breast Carcinoma
Fibroepithelial Tumors
Theranostic Applications
Genomic Applications of Immunohistochemistry: Breast Cancer Molecular Classification and Immunogenomics
Other Tumor Markers
Summary
20. Immunohistology of the Nervous System
Overview
Clinical and Radiologic Perspective of Lesions
Nonneoplastic Brain Lesions
Tumors of the Nervous System
Cysts of the Nervous System
Dementias
Demyelination
Epilepsy
Cortical Malformations
Pitfalls in Diagnosis
Summary
21. Immunocytology
Use of Immunocytology
Diagnostic Immunocytochemistry: Techniques
Diagnostic Immunocytochemistry: Applications
Pitfalls in Immunocytochemistry
Beyond Immunocytochemistry: Theranostic and Genomic Applications
Summary
22. Immunohistology of Pediatric Neoplasms
Overview
Biology of Antigens and Antibodies: Principal Antibodies
Specific Tumors
Summary
23. Imaging and Quantitative Immunohistochemistry
Overview
Imaging Systems
Software Algorithms
Strengths and Limitations
Clinical Guidelines
Clinical Applications
Summary
24. Molecular Anatomic Pathology: Principles, Techniques, and Application to Immunohistologic Diagnosis
General Principles of Molecular Biology
Genetic Polymorphism and Mutations (Sequence Variants)
Specimen Requirements for Molecular Testing
Technologies for Molecular Analysis
Genetic Alteration Detection Strategies
DNA-Based Tissue Identity Testing
Summary
INDEX
Copyright
Elsevier
1600 John F. Kennedy Blvd.
Ste 1600
Philadelphia, PA 19103-2899
DIAGNOSTIC IMMUNOHISTOCHEMISTRY: THERANOSTIC AND GENOMIC APPLICATIONS, SIXTH EDITION ISBN-13: 978-0-323-72172-1
Copyright © 2023 by Elsevier, Inc. All rights reserved.
No part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopying, recording, or any information storage and retrieval system, without permission in writing from the publisher. Details on how to seek permission, further information about the Publisher’s permissions policies, and our arrangements with organizations such as the Copyright Clearance Center and the Copyright Licensing Agency can be found at our website: www.elsevier.com/permissions .
This book and the individual contributions contained in it are protected under copyright by the Publisher (other than as may be noted herein).

Notice
Practitioners and researchers must always rely on their own experience and knowledge in evaluating and using any information, methods, compounds, or experiments described herein. Because of rapid advances in the medical sciences in particular, independent verification of diagnoses and drug dosages should be made. To the fullest extent of the law, no responsibility is assumed by Elsevier, authors, editors, or contributors for any injury and/or damage to persons or property as a matter of products liability, negligence or otherwise, or from any use or operation of any methods, products, instructions, or ideas contained in the material herein.
Previous editions copyrighted 2019 by Elsevier, Inc., and 2014, 2010, 2006, and 2002 by Saunders, an imprint of Elsevier.
Library of Congress Control Number: 2021948449
Executive Content Strategist: Michael Houston
Senior Content Development Manager: Kathryn DeFrancesco
Publishing Services Manager: Catherine Albright Jackson
S