Horsebalm FHP / Collinsonia canadensis PPH

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2012

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05 juin 2012

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Pharmacopée française - Préparations homéopathiques
05/06/2012

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Anglais Médecine

HORSEBALM FOR HOMOEOPATHIC PREPARATIONS COLLINSONIA CANADENSIS FOR HOMOEOPATHIC PREPARATIONS Collinsonia canadensis ad praeparationes homoeopathicas DEFINITION Dried underground organ ofCollinsonia canadensisL. Contentof tannins, expressed as pyrogallol (C: minimum 0.8 per cent 6H6O3;Mr 126.1) (dried drug). CHARACTERS Macroscopic and microscopic characters described under identification tests A and B. IDENTIFICATION A. Very hard and irregular, greyish-brown fragments, reaching up to 10 cm long and 2 cm in diameter. Upper side carrying rests of short and conical buds and clear scars of aerial stems. Underside bearing short and threadlike roots or their hollow scars. Examine the transverse section with a magnifying glass: brown suber, narrow cortical zone and whitish pith surrounded by a ring of dark, thin wooden tips. B. Reduce horsebalm to a powder (355). The powder is dark brown. Examine under a microscope usingchloral hydrate solution R.Fragments of suber with polyhedral cells; fragments of cellulose parenchyma; fragments of lignous tissue composed of woody vessels with reticulate or pitted patterns and lignous parenchyma with cells slightly and regularly lignified and pitted. Examine under a microscope, using a solution ofglycerol R per cent (50V/V).Small, kidney-shaped starch granules, ovoid or oblong reaching up to 40 µm long. C. Thin-layer chromatography (72.2.2). Test solution.Add 30 mL of ethanol(65 per centV/V)R 3 g of powdered drug (355). Heat to under a reflux condenser on a water-bath for 15 min. Allow to cool. Filter. Reference solution.Dissolve 20 mg ofasiaticoside Rand 10 mg ofmadecassoside Rin 10 mL ofmethanol R. Plate:TLC silica gel plate R. Mobile phase:water R, methanol R, glacial acetic acid R, methylene chloride R (2:3:8:15 VV//V/V). Application: 20 µL, as bands. ____________________________ The General Chapters and General Monographs of the European Pharmacopoeia and Preamble of the French Pharmacopoeia apply. F r e n c h P h a r m a c o p o e i a 2 0 0 7

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