Induction of cytotoxicity of Pelagia noctilucavenom causes reactive oxygen species generation, lipid peroxydation induction and DNA damage in human colon cancer cells

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2011

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Ayed Yosra , Boussabbeh Manel , Zakhama Wiem , Bouaziz Chayma , Abid Salwa , Bacha , Bacha Hassen

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biomed

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The long-lasting and abundant blooming of Pelagia noctiluca in Tunisian coastal waters compromises both touristic and fishing activities and causes substantial economic losses. Determining their molecular mode of action is, important in order to limit or prevent the subsequent damages. Thus, the aim of the present study was to investigate the propensity of Pelagia noctiluca venom to cause oxidative damage in HCT 116 cells and its associated genotoxic effects. Results Our results indicated an overproduction of ROS, an induction of catalase activity and an increase of MDA generation. We looked for DNA fragmentation by means of the comet assay. Results indicated that venom of Pelagia noctiluca induced DNA fragmentation. SDS-PAGE analysis of Pelagia noctiluca venom revealed at least 15 protein bands of molecular weights ranging from 4 to 120 kDa. Conclusion Oxidative damage may be an initiating event and contributes, in part, to the mechanism of toxicity of Pelagia noctiluca venom.

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Publié par

biomed

Publié le

01 janvier 2011

Nombre de lectures

Langue

English

Ayedet al.Lipids in Health and Disease2011,10:232 http://www.lipidworld.com/content/10/1/232

R E S E A R C HOpen Access Induction of cytotoxicity ofPelagia noctiluca venom causes reactive oxygen species generation, lipid peroxydation induction and DNA damage in human colon cancer cells 1 11 11 1,2* Yosra Ayed , Manel Boussabbeh , Wiem Zakhama , Chayma Bouaziz , Salwa Abidand Hassen Bacha

Abstract Background:The longlasting and abundant blooming ofPelagia noctilucain Tunisian coastal waters compromises both touristic and fishing activities and causes substantial economic losses. Determining their molecular mode of action is, important in order to limit or prevent the subsequent damages. Thus, the aim of the present study was to investigate the propensity ofPelagia noctilucavenom to cause oxidative damage in HCT 116 cells and its associated genotoxic effects. Results:Our results indicated an overproduction of ROS, an induction of catalase activity and an increase of MDA generation. We looked for DNA fragmentation by means of the comet assay. Results indicated that venom of Pelagia noctilucainduced DNA fragmentation. SDSPAGE analysis ofPelagia noctilucavenom revealed at least 15 protein bands of molecular weights ranging from 4 to 120 kDa. Conclusion:Oxidative damage may be an initiating event and contributes, in part, to the mechanism of toxicity of Pelagia noctilucavenom. Keywords:Pelagia noctiluca, Jellyfish, Venom, Cytotoxicity, Oxidative stress, DNA fragmentation

Background Pelagia noctiluca[1] (family Pelagiidae, Semaestomeae, Scyphozoa) is a highly venomous jellyfish species [2], widely distributed in different parts of the Mediterra nean Sea [2,3] and in the Atlantic Ocean [5].Pelagia noctiluca(P. noctulica) has not caused human fatalities, but in spite of this, it can have profound ecological and socioeconomic consequences when it appears in huge numbers during outbreaks [24]. As a matter of fact, events of massive occurrence ofP. noctulicacan be detrimental to aquaculture causing mortality of caged fish [6], to tourism by curtailing bath ing activities [7], as for fishing activities, since in several cases, it was impossible to separate the biomass of medusae from fishes [8].

* Correspondence: hassen.bacha@fmdm.rnu.tn 1 Laboratory for Research on Biologically Compatible Compounds, Faculty of Dentistry, Rue Avicenne, 5019 Monastir, Tunisia Full list of author information is available at the end of the article

One of the most distinctive aspects of jellyfish physiol ogy is related to its biologically active components and organelles contained in specialized cells called nemato cysts. They are located along the tentacles and body. These organelles contain toxins and discharge their con tent upon an appropriate stimulation [9]. The venom of P. noctulicais of protein nature and contains peptides. It is antigenic and possesses dermonecrotic and hemoly tic properties [10]; Cytolytic and neurotoxic effects have also been shown by several biological assays. The hae molytic effect is the most well studied activities of the venom [1114]. Electrophoretical analyses recognized eight different fractions, distinguished by molecular mass [15]. The protein nature of venom [16] was further con firmed by Mariottini et al. [17].P. noctilucavenom also caused an increase of ATP levels in treated cells fol lowed by a moderate decrease [17]. This is an atypic response since most toxicity studies reported decrease

© 2011 Ayed et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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