Immunohistochemical localization of integrin alpha V beta 3 and osteopontin suggests that they do not interact during embryo implantation in ruminants

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2004

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Kimmins Sarah , Lim Hai , Maclaren

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biomed

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biomed

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01 janvier 2004

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English

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1 Mo

It has been suggested that trophoblast attachment requires co-expression of integrin alpha V beta 3 and its ligand osteopontin at the fetal-maternal interface. Until now the expression patterns of integrin alpha V beta 3 and osteopontin in the pregnant bovine uterus were unknown. The objectives of this study were to localize integrin alpha V beta 3 and osteopontin in bovine and sheep endometrium during the periimplantation period and to compare the distribution patterns using antibodies that had not yet been tested in sheep. Methods Cell compartments within endometrial tissue sections were scored for immunohistochemical staining intensity and data were analyzed to determine the effects of day of pregnancy or cycle. Results In pregnant bovine endometrium, integrin alpha V beta 3 was detected in luminal epithelium, stroma, myometrium and smooth muscle. A strong band of immunoreactivity was observed in the subepithelial stroma of intercaruncular regions, but there was reduced reactivity in the caruncles and glands. Bovine trophoblast did not express integrin alpha V beta 3 at any stage of pregnancy. In ovine endometrium a different pattern of staining for integrin alpha V beta 3 was observed. Reactivity was not present in the luminal epithelium or trophoblast. There was strong staining of the deep glands and no reactivity in the superficial glands. Osteopontin distribution was similar for sheep and cattle. For both species, apical staining was present on the luminal epithelium and glands and on embryonic tissues. Conclusion In ruminants, integrin alpha V beta 3 and osteopontin do not co-localize at the fetal-maternal interface indicating that these proteins could not interact to facilitate embryo attachment as has been proposed in other species.

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Publié par

biomed

Publié le

01 janvier 2004

Nombre de lectures

Langue

English

Poids de l'ouvrage

1 Mo

Pga e 1fo1 (3apegum nr bet nor foaticnoitrup esopprods)ReveBuctiygaoioldncodnEgylonori

Introduction receptive endometrium are just now being explored. Pla-Comparative studies of placentation in domestic animals centation in ruminants is categorized as non-invasive as show that implantation is similar among these species [1- chorionic cell migration into maternal tissue is restricted 4]. However differences in the molecular properties of to the luminal epithelium [5,6]. The preimplantation

Bio Med Central

Address: 1 Institut de Génétique et de Biologie Molé culaire et Cellulaire, CNRS – INSERM – Université Lo uis Pasteur, B.P. 16367404 Illkirc h, Strasbourg, France and 2 Department of Plant and Animal Sciences , 58 River Road, Nova Scotia Agricultural College, P.O. Box 550, Truro, Nova Scotia, B2N 5E3, Canada Email: Sarah Kimmins - sarahk@igbmc.u-stra sbg.fr; Hai Choo Lim - hsmith@nsac.ns.ca; Leslie A MacLaren* - lmaclaren@nsac.ns.ca * Corresponding author

Research Open Access Immunohistochemical localization of integrin alpha V beta 3 and osteopontin suggests that they do not interact during embryo implantation in ruminants Sarah Kimmins 1 , Hai Choo Lim 2 and Leslie A MacLaren* 2

Abstract Background: It has been suggested that trophoblas t attachment requires co-expression of integrin alpha V beta 3 and its ligand osteopontin at the fetal-maternal interface. Until now the expression patterns of integrin alpha V beta 3 an d osteopontin in the pregnant bovine uterus were unknown. The objectives of this study were to lo calize integrin alpha V beta 3 and osteopontin in bovine and sheep endometrium during the periimpl antation period and to compare the distribution patterns using antibodies that had not yet been tested in sheep. Methods: Cell compartments within endometria l tissue sections were scored for immunohistochemical staining inte nsity and data were analyzed to determine the effects of day of pregnancy or cycle. Results: In pregnant bovine endometrium, integrin alpha V beta 3 was detected in luminal epithelium, stroma, myometrium and smooth mu scle. A strong band of immunoreactivity was observed in the subepithelial stroma of intercar uncular regions, but ther e was reduced reactivity in the caruncles and glands. Bovine trophoblast did not express inte grin alpha V beta 3 at any stage of pregnancy. In ovine endometriu m a different pattern of staining for integrin alpha V beta 3 was observed. Reactivity was not pres ent in the luminal epithelium or trophoblast. There was strong staining of the deep glands and no reactivity in the superficial glands. Osteopontin distribution was similar for sheep and cattle. For both species, apic al staining was present on the luminal epithelium and glands and on embryonic tissues. Conclusion: In ruminants, integrin alpha V beta 3 and osteopontin do not co-localize at the fetal-maternal interface indicating that these proteins could not interact to facilitate embryo attachment as has been proposed in other species.

Published: 28 April 2004 Received: 20 December 2003 Reproductive Biology and Endocrinology 2004, 2 :19 Accepted: 28 April 2004 This article is available from: http://www.rbej.com/content/2/1/19 © 2004 Kimmins et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copy ing and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.

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