Die Reorganisation der Fascia dentata der Ratte nach entorhinaler Denervierung [Elektronische Ressource] : eine regulatorische Rolle für das Chondroitinsulfat Proteoglykan NG2 / von Doris Dehn

pages

English

Documents

2006

Écrit par
Doris Hayman

Publié par
goethe_universitat_frankfurt_am_main

Lire un extrait

Obtenez un accès à la bibliothèque pour le consulter en ligne En savoir plus

Découvre YouScribe et accède à tout notre catalogue !

Je m'inscris

Découvre YouScribe et accède à tout notre catalogue !

Je m'inscris

pages

English

Documents

2006

Lire un extrait

Obtenez un accès à la bibliothèque pour le consulter en ligne En savoir plus

Publié par

goethe_universitat_frankfurt_am_main

Publié le

01 janvier 2006

Nombre de lectures

Langue

English

Poids de l'ouvrage

14 Mo

Die Reorganisation der Fascia dentata der Ratte nach entorhinaler Denervierung: eine regulatorische Rolle für das Chondroitinsulfat Proteoglykan NG2 Dissertation zur Erlangung des Doktorgrades der Naturwissenschaften vorgelegt beim Fachbereich Biowissenschaften der Johann Wolfgang Goethe-Universität in Frankfurt am Main von Doris Dehn aus Hattingen Frankfurt 2005 (D30) Vom Fachbereich Biowissenschaften der Johann Wolfgang Goethe-Universität als Dissertation angenommen. Dekan: Professor Dr. Rüdiger Wittig Gutachter: Professor Dr. Herbert Zimmermann Professor Dr. Thomas Deller Datum der Disputation: The reorganization of the rat dentate gyrus following entorhinal denervation: a regulatory role for the chondroitin sulfate proteoglycan NG2 Dissertation submitted to the Faculty of Biological Sciences of the Johann Wolfgang Goethe-University of Frankfurt/Main, Germany for the degree of Doctor of Natural Sciences (doctor philosphiae naturalis, Dr. phil.nat.) presented by Doris Dehn (Hattingen/Ruhr) Frankfurt/Main, 2005 Accepted by the Faculty of Biological Sciences of the Johann Wolfgang Goethe-University Frankfurt/Main, Germany Dean: Professor Dr. Rüdiger Wittig Referees: Professor Dr. Herbert Zimmermann Professor Dr.

Voir

Publié par

goethe_universitat_frankfurt_am_main

Publié le

01 janvier 2006

Langue

English

Poids de l'ouvrage

14 Mo

Gesundheit





Die Reorganisation der Fascia dentata der Ratte

nach entorhinaler Denervierung:

eine regulatorische Rolle für das Chondroitinsulfat Proteoglykan NG2

Dissertationzur Erlangung des Doktorgrades der Naturwissenschaften

vorgelegt beim Fachbereich Biowissenschaften der Johann Wolfgang Goethe-Universität in Frankfurt am Main

von Doris Dehn aus Hattingen 



Frankfurt 2005 (D30)



Vom Fachbereich Biowissenschaften der

Johann Wolfgang Goethe-Universität als Dissertation angenommen.



Dekan:



Gutachter:



Datum der Disputation:

Professor Dr. Rüdiger Wittig

Professor Dr. Herbert Zimmermann

Professor Dr. Thomas Deller





The reorganization of the rat dentate gyrus

following entorhinal denervation:

a regulatory role for the chondroitin sulfate proteoglycan NG2

Dissertation

submitted to the Faculty of Biological Sciences

of the Johann Wolfgang Goethe-University of Frankfurt/Main, Germany

for the degree of

Doctor of Natural Sciences

(doctor philosphiae naturalis, Dr. phil.nat.)

presented by

Doris Dehn

(Hattingen/Ruhr)



Frankfurt/Main, 2005



Accepted by the Faculty of Biological Sciences

of the Johann Wolfgang Goethe-University

Frankfurt/Main, Germany



Dean:



Referees:



Oral examination:

Professor Dr. Rüdiger Wittig

Professor Dr. Herbert Zimmermann

Professor Dr. Thomas Deller







'What is this,' said the Leopard, 'that is so 'sclusively dark,

and yet so full of little pieces of light?'

Rudyard Kipling, How the leopard got his spots



ABSTRACT



INTRODUCTION

REORGANISATION PROCESSES AFTER CENTRAL NERVOUS SYSTEM INJURYAXONAL SPROUTING AFTER ENTORHINAL CORTEX LESIONMOLECULAR REGULATION OF SPROUTINGCHONDROITINSULFATE PROTEOGLYCANS(CSPGS) AMEMBER OF THECSPGFAMILY: NG2 AIMS OF THE STUDY

MATERIALS AND METHODS

ANIMALS,SURGICAL PROCEDURES,AND TISSUE PREPARATIONANIMALSENTORHINAL CORTEX LESIONTISSUE PREPARATION

CONTROL OF LESION QUALITYFLUORO-JADEBANDACHESTAINING ON FRONTAL SECTIONS OF RAT DENTATE GYRUSCOMBINED LASERMICRODISSECTION(LMD)AND QUANTITATIVERT-PCR (QPCR) FLOW CHART OF THE COMBINEDLMDAND QPCRPROTOCOLPROTOCOL FORRNAISOLATION USING THELMDANDRNEASY®MICROKIT (QUIAGEN)/TAQMANREVERSETRANSCRIPTIONREAGENTS(APPLIEDBIOSYSTEMS) QUANTITATIVE REVERSE TRANSCRIPTION-PCR (QPCR) IMMUNOHISTOCHEMISTRYSTAINING PROTOCOLSBUFFER SOLUTIONS

TRANSMISSION ELECTRON MICROSCOPYBROMO-DEOXYURIDINE INJECTIONDIGITAL PROCESSING OF ILLUSTRATIONS

RESULTS

10

11

121416181820

22

2222 22 23

23 26 2728

29 31 3233 35 363636

37

AMOUNT OFNG2MRNAIS SIGNIFICANTLY INCREASED IN THE DENERVATED OUTER MOLECULAR LAYER 37NG2-POSITIVE CELLS ARE REGULARLY DISTRIBUTED IN THE DENTATE GYRUS OF CONTROL RATS 39STAINING OFNG2-POSITIVE CELLS IS ENHANCED IN THE DENERVATED OUTER MO CULA 39LE R LAYER TOF THE DENERVATED OUTER MOLECULAR LAYER IS ENRICHED WITHHE NEUROPIL NG2 FOLLOWING ENTORHINAL CORTEX LESION 42ELECTRON MICROSCOPY OFNG2-POSITIVE CELLS IN THE OUTER MOLECULAR LAYER: IMMUNOPRECIPITATE IN THE EXTRACELLULAR MATRIX SURROUNDING GLIAL AND NEURONAL PROFILES 44NG2-POSITIVE CELLS DO NOT EXPRESS ASTROGLIAL,MICROGLIAL,AND ADULT OLIGODENDROGLIAL MARKERS 47ASUBPOPULATION OFNG2-POSITIVE CELLS IS GENERATED POSTLESION IN THE OUTER OLECULAR LAY 50M ER 

DISCUSSION

52

SUMMARY OF RESULTS 52METHODOLOGICAL CONSIDERATIONS 52COMBINED LASERMICRODISSECTION AND QUANTITATIVERT-PCR 53 IMMUNOHISTOCHEMISTRY 55 DOESNG2AFFECT AXONS WITHIN THE ZONE OF DENERVATION? REGULATION OF BRANCHING AND AXONAL COMPLEXITY 56DOESNG2AFFECT AXONS OUTSIDE THE ZONE OF SPROUTING? REGULATION OF A LAYER-SPECIFIC SPROUTING RESPONSE 58SECRETION OF CLEAVEDNG2INTO THE ZONE OF SPROUTING 59REGULATION OFECLINDUCED AXONAL GROWTH:THE ROLE OF THE EXTRACELLULAR MATRIX 60NG2-POSITIVE CELLS:A FOURTH GLIAL CELL TYPE IN THE BRAIN OF ADULT RATS? 60

SUMMARY AND CONCLUSION

64

REFERENCE LIST

CURRICULUM VITAE

PUBLICATIONS

ACKNOWLEDGEMENT

ZUSAMMENFASSUNG (DEUTSCH)

LEBENSLAUF



65

79

80

83

84

93

Voir