Application of DNA marker systems to test for genetic imprints of habitat fragmentation in Juniperus communis L. on different spatial and temporal scales [Elektronische Ressource] : integration of scientific knowledge into conservation measures / vorgelegt von Inga Maria Michalczyk

Application of DNA marker systems
to test for genetic imprints of habitat fragmentation in
Juniperus communis L.
on different spatial and temporal scales
***
Integration of scientific knowledge into conservation measures
Dissertation
zur Erlangung des Doktorgrades
der Naturwissenschaften
(Dr. rer. nat.)
dem Fachbereich Biologie
der Philipps-Universität Marburg
vorgelegt von
Inga Maria Michalczyk
aus Bamberg
Marburg/Lahn, 2008 Application of DNA marker systems
to test for genetic imprints of habitat fragmentation in
Juniperus communis L.
on different spatial and temporal scales
***
Integration of scientific knowledge into conservation measures
Einsatz von DNA-Marker-Systemen
zur Überprüfung genetischer Effekte der Habitatfragmentierung auf
Juniperus communis L.
innerhalb unterschiedlicher räumlicher und zeitlicher Skalen
***
Einbindung wissenschaftlicher Erkenntnisse in Naturschutzmaßnahmen
Dissertation
zur Erlangung des Doktorgrades
der Naturwissenschaften
(Dr. rer. nat.)
dem Fachbereich Biologie
der Philipps-Universität Marburg
vorgelegt von
Inga Maria Michalczyk
aus Bamberg
Marburg/Lahn, 2008 The research pertaining to this thesis was carried out at the Department of Conservation
Biology at the Philipps-University of Marburg from January 2005 to September 2008 under
the supervision of Prof. Dr. Birgit Ziegenhagen. Vom Fachbereich Biologie
der Philipps-Universität Marburg als Dissertation am 18. September 2008 angenommen.
Erstgutachterin: Prof. Dr. Birgit Ziegenhagen
Zweitgutachter: Prof. Dr. Gerhard Kost
Tag der mündlichen Prüfung am 11. November 2008
Neues wagen, Positives entdecken, jeden Tag. This thesis is based on the following three publications and manuscripts. They will be referred
to in the text by the term ‘paper’ and their roman numerals. They are reviewed in chapter 2
and 3 of this thesis. After a short general introduction into the main topics, the main results of
the respective scientific analyses are given and interpreted. For detailed information about
materials and methods as well as for further results and discussions see appended
publications and manuscripts.
I Genetic support for recurrent fragmentation and founder events of juniper
populations in Central Europe
Inga M. Michalczyk, Yvonne A. Lücke, Stefan Huck and Birgit Ziegenhagen
Manuscript submitted to The Holocene.
Reviews received with the comment ‘possibly acceptable after moderate revision’.
II Characterization of highly polymorphic nuclear microsatellite loci in Juniperus
communis L.
Inga M. Michalczyk, Federico Sebastiani, Anna Buonamici, Eva Cremer, Christina
Mengel, Birgit Ziegenhagen and Giovanni G. Vendramin
Molecular Ecology Notes (2006) 6, 346-348.
III Reduction of population sizes has not yet affected genetic diversity of juniper,
Juniperus communis L.
Inga M. Michalczyk, Ronald Bialozyt, Frank Schlütz and Birgit Ziegenhagen
Manuscript submitted to Biological Conservation. Contents
Contents
Synthesis ___________________________________________________ 1
German summary – Zusammenfassung _____________________________________ 2
English summary________________________________________________________ 5
1 Introduction................................................................................................................8
2 Biogeographic history of Juniperus communis in Central Europe:
historic fragmentation effects on a large spatial scale ......................................14
3 Population genetic analysis in the Rhenish Uplands:
recent fragmentation effects on a regional scale................................................19
3.1 Development of species-specific nuclear microsatellite (nSSR) markers.......19
3.2 Validation and application of the newly developed nSSR markers.................20
3.3 Genetic diversity and differentiation of eight J. communis populations in the
Rhenish Uplands ..............................................................................................22
4 Integration of scientific knowledge into conservation measures .....................27
4.1 Evaluation of J. communis populations for nature conservation purposes.....27
4.1.1 Establishment of a ‘Leitbild’ for J. communis populations......................27
4.1.2 Evaluation of the analysed J. communis populations.............................28
4.2 Critical comments on the evaluation criteria with respect to the species
J. communis......................................................................................................31
4.3 Guidelines and recommendations for a specific restoration management .....33
4.3.1 Guidelines and recommendations for the collection of plant material....34
4.3.2 Guidelines and recommendations for the greenhouse...........................35
4.3.3 Guidelines and recommendations for plantings in the field....................35
5 Conclusions and perspectives ..............................................................................37
5.1 Imprints of habitat fragmentation in J. communis and the usability of
the scientific results from a nature conservation perspective..........................37
5.2 Perspectives for further analyses.....................................................................38
6 References ...............................................................................................................42
7 Abbreviations...........................................................................................................53 Contents
Publications and Manuscripts _________________________________ 55
I Genetic support for recurrent fragmentation and founder events of juniper
populations in Central Europe................................................................................56
II Characterization of highly polymorphic nuclear microsatellite loci in Juniperus
communis L. ...........................................................................................................77
III Reduction of population sizes has not yet affected genetic diversity of juniper,
Juniperus communis L. ..........................................................................................81
Acknowledgements – Danksagung _______________________________________ 108
Declaration ___________________________________________________________ 110
Curriculum vitae ______________________________________________________ 111 Synthesis
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