Etude de la résolution en profondeur lors de l'analyse par spectrométrie de masse des ions secondaires.

6LJQDX[
G·XQ
HQ
+$3,75(

&
VXSHUUpVROXWLRQ
$
%UXLW
'e&2192/87,21
JDXVVLHQ


35,1&,3(6

(7
1RWLRQ
0e7+2'(6
PDWULFLHOOH
(7
FRORUp
35,1&,3(6
GH
'(&2192/87,21

1752'8&7,21
GH
$
180e5,48(
(6

75$,7(0(176

180e5,48(6
'pILQLWLRQ





FRQYROXWLRQ
$
R
'e&2192/87,21

QRUPH

32,17

'(
VHV
98(
(TXDWLRQ
$1$/2*,48(
3UHPLqUH
HW
ILOWUH
VFDODLUH
IRUPDOLVPH
3URGXLW




&RQYROXWLRQ

QRQEUXLWp
'pILQLWLRQV






EUXLW


/LQpDULWp
G·XQ
HW

UpSRQVH
EODQF
LPSXOVLRQQHOOH
GH

URVH

EUXLW



&DUDFWpULVDWLRQ
,QYDULDQFH






FRQVpTXHQFHV


&DXVDOLWp
%UXLW



FRQYROXWLRQ



GH
6WDELOLWp
EUXLWpH

:LHQHU

$

'(


(TXDWLRQ
FLUFXODLUH
GH

FRQYROXWLRQ
6\VWqPHV








3URSULpWpV
&DV
GX
OD
SURGXLW

GH

FRQYROXWLRQ




GH




/D
&DUDFWpULVDWLRQ
GpFRQYROXWLRQ
IUpTXHQFH

EUXLW
FDV

JpQpUDO


%UXLW





(TXDWLRQ
([LVWHQFH
©
G·XQ
ª
LQYHUVH
X
GH
©
FRQYROXWLRQ
ª





SUREDELOLVWH
([HPSOHV
EUXLW





'HQVLWp

SUREDELOLWp
7RHSOLW]

)RQFWLRQ

GH
%UXLW
UpVROXWLRQ

*DXVVLHQQH


HW

SRLVVRQLHQ


GH

PDWULFH
GH
)RQFWLRQ
EUXLWpH
GH

UpVROXWLRQ

/RUHQW]LHQQH
UpVROXWLRQ

O·pTXDWLRQ

FRQYROXWLRQ


OD
GH
GH

)RQFWLRQ
PDWULFLHO
GH
'e&2192/87,21
UpVROXWLRQ
32,17
H[SRQHQWLHOOH
98(

/H






&DV
GLVFUHWV
R

O·LQYHUVH

GH
GLVFUHWV
FRQYROXWLRQ

Q·H[LVWH

SDV
GLVFUqWH


VROXWLRQ
&RQYROXWLRQ
SULQFLSDOH
&DV







EUXLWp
3ULQFLSH
0(7+2'(6
GH

/
&+$3,75(
/
6RPPDLUH GpWDLOOp GH FH FKDSLWUH
, /
/
*pQpUDOLWpV GHFRQYROXWLRQ HQ O•DEVHQFH GH EUXLW
•
•
•
’pFRQYROXWLRQ HQ SUpVHQFH GH EUXLW
•
•
•
•
•
/
IRUPDOLVPH GLVFUHW
•
•
•
•
•

FURLVpH

+$3,75(

21&/86,21
OD
$

'e&2192/87,21
(6


35,1&,3(6
GH
(7

0e7+2'(6
,QWURGXFWLRQ

GX

JpQpUDOLVpH


5HODWLRQV
GX
XWLOHV

HW

UqJOHV

GH
PpWKRGH
GpULYDWLRQ
OD

0LVH



SURSULpWpV
HUUHXUV

GHV

&RQGLWLRQQHPHQW

G·XQH
'e&2192/87,21
PDWULFH
&RQYHUJHQFH

$OJRULWKPH




'pILQLWLRQ
&RQWUDLQWH

GH




3UREOqPHV
&RQFOXVLRQ
PDO
GH
SRVpV
PD[LPXP







RHXYUH
,QIOXHQFH
)RXULHU
GX

EUXLW
8WLOLVDWLRQ
HW
OD
XQLFLWp

GH
/D
OD

VROXWLRQ
YDOLGDWLRQ



FRQWUDLQWHV

,7e5$7,)6


6ROXWLRQ
O·DOJRULWKPH
GHV
$OJRULWKPH
PRLQGUHV

FDUUpV
VRXVWUDFWLRQ
HW

pTXDWLRQ

QRUPDOH


&RQWUDLQWH



VXSSRUW




JpQpUDO
O·DOJRULWKPH
LWpUDWLI
GH
$OJRULWKPH
&LWWHUW

'pILQLWLRQ
/H

SUREOqPH
VXU
GH
SUpVHQWDWLRQ
PLQLPLVDWLRQ
OD
GH
GX
/DJUDQJH
G·HQWURSLH



UpJXODULVDWLRQ

GH

3ULQFLSH
3KLORVRSKLH

JpQpUDOH

GHV
HQ
SUREOqPH
GDQV
GH
GH
UpJXODULVDWLRQ








GHV
6ROXWLRQ
GH
GHV
ORL
PRLQGUHV
c
FDUUpV

GH

QRUPH
YDOLGDWLRQ
PLQLPDOH




/D

FURLVpH





GXUHV

GHV
LWpUDWLI
$/*25,7+0(6
O·DOJRULWKPH
'(
GDQV
-XVWLILFDWLRQ
0LOOHU

GH
FRQWUDLQW

GH
O·H[HPSOH

WULYLDO
VLPSOH
PDLV

KLVWRULTXH

GHV
DYHF
NDQJRXURXV
WHUPLQDOH

EUXLW






)RUPDOLVDWLRQ
3RVLWLYLWp
GX

SUREOqPH

HW
G·DPSOLWXGH
RULJLQH

GH

OD
GH
IRQFWLRQQHOOH

G·HQWURSLH


&RPSRVLWLRQ

FRQWUDLQWHV



FRQWUDLQW
$SSOLFDWLRQ
GH
j
*pQpUDOLVDWLRQ
O
O·DOJRULWKPH
D
9DQ
GpFRQYROXWLRQ




O·HVSDFH

&
/
•
•
5pVROXWLRQ GH O•pTXDWLRQ GH FRQYROXWLRQ GLVFUqWH
/D UpJXODULVDWLRQ
/D UpJXODULVDWLRQ GH 0LOOHU
/D PpWKRGH GX PD[LPXP G•HQWURSLH
5pVROXWLRQ GLUHFWH GHV SUREOqPHV UpJXODULVpV
&KRL[ GH GX SDUDPqWUH GH UpJXODULVDWLRQ
’pFRQYROXHU VDQV IRQFWLRQ GH UpVROXWLRQ
/
,QWURGXFWLRQ MXVWLILFDWLRQ GX FKRL[ G•XQH PpWKRGH LWpUDWLYH
$OJRULWKPH GH 9DQ &LWWHUW
$SSOLFDWLRQ GH FRQWUDLQWHV GXUHV
/HV DOJRULWKPHV LWpUDWLIV DYHF FRQWUDLQWHV GXUHV
•
•
•
&
175
75$,7(0(176
180(5,48(6
+$3,75(
35,1&,3(6
&
0e7+2'(6
$
(6
'e&2192/87,21
2'8&7,21

(7
/
, /
/•DQDO\VH 6,06DWWHLQW OHV OLPLWHV SK\VLTXHV TXL UHQGHQW GLIILFLOHPHQW HQYLVDJHDEOHV G•DXWUHV
DPpOLRUDWLRQVWHFKQLTXHVGH O•DSSDUHLOODJH VDQVTXH OH FR W GHYLHQQH H[RUELWDQW QRQ SDVWDQW VXU OH
SODQ ILQDQFLHU HW GH OD FRPSOH[LWp GH PLVH HQ RHXYUH GH O•DQDO\VH TXH VXU FHOXL GX WHPSV GH PHVXUH
,O HVW SRVVLEOH GH FRQVWUXLUH GHV FRORQQHV SULPDLUHV FDSDEOHV GH IRFDOLVHU GHV LRQV GH WUqV IDLEOH
pQHUJLH TXHOTXHV FHQWDLQHV G•H9 PDLV OD UpGXFWLRQ GX WDX[ G•pURVLRQ LQKpUHQW j OD IDLEOH pQHUJLH
LQFLGHQWH UHQG OHV WHPSV GH PHVXUH WUqV ORQJV DYHF WRXV OHV LQFRQYpQLHQWV TXH FHOD VXSSRVH HQ
WHUPHV GH VWDELOLWp GX FRXUDQW SULPDLUH
1RXV YHUURQV TXH OHV PpWKRGHV QXPpULTXHV SHUPHWWHQW G•REWHQLU GDQV FHUWDLQV FDV XQH
DPpOLRUDWLRQ WUqV VHQVLEOHV GH OD UpVROXWLRQ HQ SURIRQGHXU j SDUWLU G•XQH PHVXUH HIIHFWXpH GH PDQLqUH
URXWLQLqUH ,O Q•HVW ELHQ V U SDVTXHVWLRQ GH UHPSODFHU XQH ERQQH PHVXUH SDU XQ FDOFXO QXPpULTXH
/D ILDELOLWp HW OD TXDOLWp GH OD PHVXUH GH GpSDUW UHVWH OD FRQGLWLRQ HVVHQWLHOOH SRXU XQ ERQ UpVXOWDW
WRXW FRPPH O•DPpOLRUDWLRQ GH O•LQVWUXPHQWDWLRQ UHVWH XQH YRLH SULYLOpJLpH YHUVO•DPpOLRUDWLRQ XOWLPH
GH OD UpVROXWLRQ HQ SURIRQGHXU /D GpFRQYROXWLRQ YLHQW V•DMRXWHU DX[ SHUIRUPDQFHV LQVWUXPHQWDOHV HW
SHUPHW GH WLUHU OH PD[LPXPGH UpVROXWLRQ G•XQ SURILO H[SpULPHQWDO ,OHVWSDU DLOOHXUVWUqVKDVDUGHX[
GH UpDOLVHU GHV WUDLWHPHQWV QXPpULTXHVVXUOHVUpVXOWDWVGH PDXYDLVHV PDQLSXODWLRQV
,O IDXW JDUGHU j O•HVSULW TXH OD GpFRQYROXWLRQ UHVWH XQ PR\HQ G•REWHQLU GH PHLOOHXUHV
UpVROXWLRQVHQ SURIRQGHXU DYHF O•DSSDUHLOODJH GRQW RQ GLVSRVH VDQVTX•LO VRLW QpFHVVDLUH G•LQYHVWLUGDQV
XQ QRXYHO DQDO\VHXU LRQLTXH (OOH SHUPHW pJDOHPHQW GDQV XQH RSWLTXH RX OHV PHVXUHV GRLYHQW rWUH
DXWRPDWLVpHV GH UpDOLVHU GHV PHVXUHV SOXV SHUIRUPDQWHV GDQV XQ PLQLPXP GH WHPSV F•HVW j GLUH
VDQVPRQRSROLVHU O•DSSDUHLO SDU OHVUpJODJHVH[WUrPHPHQW PLQXWLHX[ QpFHVVDLUHVjO•REWHQWLRQ GH OD
PHLOOHXUH UpVROXWLRQ HQ SURIRQGHXU SRVVLEOH ,O VHUD ELHQ VU WRXMRXUVSRVVLEOH G•HIIHFWXHU FHV
UpJODJHVPLQXWLHX[ SRXU REWHQLU OD PHLOOHXUH UpVROXWLRQ LQVWUXPHQWDOH SXLVGH FRXSOHU FHWWH DQDO\VH
DYHF XQ WUDLWHPHQW QXPpULTXH TXL DPpOLRUHUD HQFRUH OHV SHUIRUPDQFHV 2Q SHXW GRQF SHQVHU TXH OD
GpFRQYROXWLRQ VHUD OH GHUQLHU PDLOORQ GDQV OH SURFHVVXV G•REWHQWLRQ GH OD UpVROXWLRQ HQ SURIRQGHXU
XOWLPH GH O•DQDO\VH 6,06 ORUVTX•HOOH VHUD PLVH HQ RHXYUH VXU GHV SURILOV REWHQXV SDU GHV DSSDUHLOV TXL
DXURQW DWWHLQW OH GHJUp XOWLPH V•LO HVW MDPDLV SRVVLEOH GH OH GpILQLU GH VRSKLVWLFDWLRQ
1pDQPRLQVOD GpFRQYROXWLRQ UHVWH XQH RSpUDWLRQ GpOLFDWH TX•LO QH IDXW SDVPHWWUH HQ RHXYUH
VDQV SUpFDXWLRQV VRXV SHLQH G•REWHQLU GHV UpVXOWDWV DEHUUDQWV ,O H[LVWH XQ JUDQG FKRL[ G•DOJRULWKPHV
GH GpFRQYROXWLRQ TXL SUpVHQWHQW GHV VSpFLILFLWpV SURSUHV HW GRQW OH FKRL[ Q•HVW SDV pYLGHQW D SULRUL
/H SOXV JUDQG GDQJHU LQKpUHQW j OD GpFRQYROXWLRQ HVW G•DERXWLU j XQ UpVXOWDW PDWKpPDWLTXHPHQW
FRUUHFW PDLV SK\VLTXHPHQW LUUpDOLVWH FH TXL QpFHVVLWH GH ELHQ FRQWU{OHU OH SURFHVVXV GH UHVWLWXWLRQ HW